OF ORGANIC SUBSTANCES BY THIN LAYER CHROMATOGRAPHIC METHOD .
Keywords:
Key words: (TLC), silica gel, aluminum oxide, theophedrine tablets, mixture , cellulose , kieselguhr , polyamide.Abstract
Annotation: An important advantage of chromatographic methods is speed, i.e.
express method. In chemical analysis, there are more types of surface chromatography
performed on paper or a thin layer of sorbent.
Thin layer chromatography - substances in the mixture are separated due to the
different distribution of the mobile eluent (mixture of solvents) and the thin layer of
the sorbent uniformly attached to the plate [2,3,7]. To perform chromatography on a
thin layer of a sorbent, a sorbent-plaster mixture is evenly spread on the surface of a
glass or foil plate using a special device and dried. A starting line is drawn with a soft
pencil at a distance of 1.5 cm from the bottom edge of the plate . A small drop of the
test solution and the standard solution of the substance to be detected is dropped in a
thin capillary device at a distance of 1.0-1.5 cm from the start line. At the bottom of
the plate, a glass (column) with a 1 cm thick eluent is placed vertically, and the mouth
of the glass is closed[4,6,8]. The eluent moistens the sorbent and moves to the finish
line by dissolving the substances brought to the starting-line.
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